The H-Tyr-pNA compound is commonly utilized in chemical synthesis as a substrate for specific enzymatic reactions. This synthetic compound serves as a substrate for enzymes, such as proteases, which cleave peptide bonds at the C-terminal side of tyrosine residues. By incorporating H-Tyr-pNA into reaction mixtures, researchers can monitor enzyme activity through colorimetric or spectrophotometric assays. The cleavage of H-Tyr-pNA by the enzyme releases p-nitroaniline (pNA), leading to a measurable absorbance shift at a specific wavelength. This substrate's application in chemical synthesis enables the precise quantification of enzyme kinetics and facilitates the study of protease activity in various biological processes.
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